The effect of temperature change upon inclusion-containing renal tumor cells of leopard frogs.

at Day 7. Previously 1 frog with a renal tumor had been studied 7 days post hibernât ion; thus a total of 8 animals were examined. Light microscopio examination of the tumors showed that the amount of cellular debris increased within the lumina of the tumor tubules during this period. Electron microscopic observa tions showed that the lumina were filled with cellular debris as well as incomplete and complete viruses. Observations on the effect of increased temperature on hibernating frogs resulting in the destruction of tumor cells which contain viral inclusions is discussed.